Expression of lipid synthetic enzymes is markedly decreased in Akt1-/-; Akt2+/- mice. Relative expression of Aldoc, Fads1 and Elovl5 mRNA in mammary glands from Akt knockout mice at day 9 of lactation (n = 4 for each genotype). Average expression values normalized to cytokeratin 18 ± standard error of the mean are shown. Statistical differences in expression were calculated by comparing each group with Akt1+/+;Akt2+/+ mice except the indicated P values shown between Akt1-/-;Akt2+/+ mice and Akt1-/-;Akt2+/- mice. *P < 0.05. **P < 0.01. ***P < 0.001.