Figure 5From: Association of invasion-promoting tenascin-C additional domains with breast cancers in young women Direct effects of tenascin-C isoform expression on tumour cell invasion. (a) Monitoring tumour cell invasion in real time using a modified Boyden chamber assay in (i) MCF-7, (ii) T-47 D, (iii) MDA-MB-231 and (iv) GI-101 cell lines transfected with three different TNC isoform constructs and the vector control; and (v) invasion of MCF-7 cells transfected with TNC-L and TNC-14/AD1/16 constructs. In all cases, data points represent the mean of six replicates representing three separate transfections; error bars shown. (b) Quantitative RT-PCR of total TNC transcripts (exons 17/18) from cells transfected with TNC constructs.Back to article page