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Table 1 Main features of ImmunoRatio web application

From: ImmunoRatio: a publicly available web application for quantitative image analysis of estrogen receptor (ER), progesterone receptor (PR), and Ki-67



Analytical principle

Analyzes immunostained slides (ER, PR, Ki-67) using color deconvolution [12] for stain separation and adaptive IsoData algorithm [21] for thresholding.

Users can analyze either the whole image or a region of interest.

Hardware and software requirements

Runs within the web browser, requiring no additional program or plugin installations. Is compatible with all modern web browsers and operating systems.

Compatibility with different microscope setups

Adapts to various combinations of microscope objective lenses, phototubes, and camera resolutions.

Supports most existing camera models and image formats (JPEG, JPEG2000, TIFF, BMP, PNG).

Users can define optimal camera brightness and contrast settings with the Camera Adjustment Wizard.


Users can calibrate the application to match with their own visual cell counting data.

Usage modes

Includes a basic mode for introductory analyses and a full-featured mode.

  1. ER, estrogen receptor; PR, progesterone receptor.