Figure 7

MYB overexpression prevents differentiation-inducing agents-induced apoptosis in a BCL2 -dependent manner. (a) MCF-7 cells were treated with the indicated doses of sodium butyrate (NaBu) for 24 hours before being assayed with TUNEL, which was quantified by flow cytometry. (b) MCF-7 cells stably overexpressing WT-MYB, CT3-MYB, or vector, or control parental, were treated with 10 mM or 100 mM NaBu for 24 hours prior to TUNEL assay. (c) MCF-7 cells stably overexpressing WT MYB, or control vector, were transiently transfected with siRNAs targeting BCL2. They were then treated with 0 or 100 mM NaBu for 24 hours before being assayed with TUNEL. Standard deviation is represented by error bars (n = 6).