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Figure 6 | Breast Cancer Research

Figure 6

From: MYBsuppresses differentiation and apoptosis of human breast cancer cells

Figure 6

MYB overexpression in HC11 cells prevents differentiation and growth arrest. (a) HC11 cells were stably transduced with empty vector, WT-MYB, or CT3-MYB, as in Figure 5. Western blot with anti-MYB Ab and anti-HA Ab confirmed the expression of exogenous and HA-tagged MYB proteins. A ß-tubulin loading control is also shown. (b) MYB overexpression blocks lipid accumulation associated with differentiation. Cells were stained with Nile Red at the various stages of differentiation shown, and analyzed by flow cytometry. Standard deviation is indicated by error bars. (c) HC11 cells overexpressing WT or CT3-MYB continue to proliferate in differentiation-inducing conditions. HC11 cells as indicated were grown in complete media for two days, after which epidermal growth factor (EGF) was removed for 24 hours and subsequent lactogenic hormones were added to the medium each day Standard deviation is indicated by error bars (n = 3).

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