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Figure 3 | Breast Cancer Research

Figure 3

From: MYBsuppresses differentiation and apoptosis of human breast cancer cells

Figure 3

MYB knockdown sensitizes MCF-7 to differentiation-inducing agents. (a) Vitamin E succinate (VES) was used to treat MCF-7 cells with a doxycycline (Dox)-inducible shRNA targeting MYB [19] MCF-7 cells grown on a glass coverslip, and treated with or without 5 mg/mL Dox for 24 hours before exposure to VES for three days, were then stained with Nile Red (red) for lipid vesicles and 4',6-diamidino-2-phenylindole (DAPI) (blue) for DNA detection. (b) Sodium butyrate (NaBu) was used to treat MCF-7 cells with the Dox-inducible shRNA targeted against MYB, as in (a). Results are indicative of at least two independent experiments. (c) Flow cytometric analysis of TUNEL staining for apoptotic cells. MCF-7 cells stably transfected with inducible shRNA treated for 24 hours with or without Dox, were grown in the presence or absence of 1 mM NaBu for a further 24 hours, and were then assayed for apoptosis using TUNEL. Standard deviation is represented by error bars.

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