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Figure 3 | Breast Cancer Research

Figure 3

From: Dissecting the transcriptional networks underlying breast cancer: NR4A1 reduces the migration of normal and breast cancer cell lines

Figure 3

NR4A1 reduces the migratory ability of MCF-10A cells. (a) A wound was scratched in a confluent monolayer of control and MCF10A-NR4A1 cells. The edge of the wound was monitored over 24 hours and pictures of the same fields were taken at 0, 17 and 24 hours. Scale bars: 300 μm. (b) Percentage migration. (c) To confirm the migration phenotype, cells were plated on the top chamber of transwells in the absence of EGF and were allowed to migrate overnight towards medium supplemented with EGF present in the lower chamber. (d) To compare the proliferation rate of control and MCF10A-NR4A1 cells, the MTT assay was performed. (e) The Annexin V assay was performed to detect apoptosis in the two cell lines. Graphs show the mean ± standard deviation of representative experiments out of at least n = 3. *P < 0.001, **P < 0.005. PI, propidium iodide.

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