Tamoxifen enhances the cytotoxic effects of nelfinavir in breast cancer cells

Breast Cancer Research

Table 1 Quantitative analysis of apoptotic and necrotic cells after tamoxifen and nelfinavir treatment

Cell line	LL (%)	UL (%)	UR (%)	LR (%)
T47D
Control	98.78	0.44	0.77	0.03
TAM 6 μg/ml	92.71	4.42	2.71	0.17
NFV 6 μg/ml	95.50	2.17	2.31	0.03
TAM/NFV	11.18	43.05	44.44	1.34
MCF7
Control	88.04	3.10	7.52	1.33
TAM 6 μg/ml	83.05	7.16	6.89	2.90
NFV 6 μg/ml	92.80	2.46	3.80	0.95
TAM/NFV	63.68	14.63	19.48	2.21

The relative cell distributions among the quadrants shown in Figure 2 were analysed using the BectonDickinson CellQuest software. LL, lower left (unstained; viable); UL, upper left (annexin-positive; apoptotic); UR, upper right (annexin-positive and propidium iodide-positive; apoptotic/necrotic); LR, lower right (propidium iodide permeable; necrotic); TAM, tamoxifen; NFV, nelfinavir.

ISSN: 1465-542X