Figure 3
From: Tamoxifen enhances the cytotoxic effects of nelfinavir in breast cancer cells
Combination of nelfinavir and tamoxifen enhances apoptosis in breast cancer cells. The breast cancer cell lines MCF7 and T47 D were incubated with the indicated nelfinavir (NFV) and tamoxifen (TAM) concentrations either alone at 6 μg/ml or in combination (6 μg/ml NFV plus 6 μg/ml TAM), and after 48 hours of incubation were analysed by FACScan analysis for the occurrence of apoptosis (FITC-annexin binding) and necrosis (propidium iodide permeability). FL-1 setting (propidium iodide; PJ), 575 nm; FL-2 setting (FITC), 530 nm.