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Figure 4 | Breast Cancer Research

Figure 4

From: RNA interference (RNAi) screening approach identifies agents that enhance paclitaxel activity in breast cancer cells

Figure 4

Drug combinations to enhance cell death of TNBC cell lines. A. Twenty-two triple-negative cell lines were each seeded in 96-well plates. The next day cells were treated with vehicle control or paclitaxel (0.3 to 30 nM). IC50 values for each cell line were generated based on the median-effect plot from three independent experiments. IC50 values represent the inhibitor concentration required for a 50% reduction in cell viability relative to vehicle-treated controls. Error bars represent standard deviation of four replicates from three independent experiments. B. Cell lines were seeded in 96-well plates and treated with single agents (IC50 values) or a combination of drugs (CCT007093 + paclitaxel or mithramycin + paclitaxel) of the IC50 concentrations of each drug (1:1 ratio) serial-diluted (IC50-IC25-IC12.5). Combination index (CI) values were calculated using the Chou-Talalay method with CalcuSyn software (Biosoft). CI values significantly > 1 are antagonistic, not significantly different than 1 are additive, and values < 1 are synergistic. Error bars represent standard deviation of quadruplicates from three independent experiments.

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