Figure 1

shRNA screen to identify paclitaxel sensitizers. A. The overlay of druggable genome libraries (Qiagen and Open Biosystems) and genes deregulated in breast cancer resulted in 428 candidate druggable genes. B. Reproducibility of shRNA screen by correlation of the effect of shRNAs on cell growth compared to non-silencing shRNA in vehicle-treated plates of two replicate experiments. Spearman correlation coefficient, r = 0.89. C. Each shRNA was scored for the level of paclitaxel sensitivity using the sensitivity index (SI) as described in Materials and Methods. The SI score ranges from -1 to 1. Positive significant SI scores indicate sensitization and negative significant SI scores indicate antagonism. The scatter plot of all shRNAs is shown in rank order. The dashed lines indicate the relative threshold of significant drug sensitivity.