Figure 7

Flow-cytometry analysis and relative proportions of cell types. (a) Flow-cytometry analysis showing the distribution of Lin^-CD29^lo for the expression of the surface markers CD24 and CD61. Mammary glands from 10-week-old FVB mice were included as a control for the expression of these cell-surface markers and analysis by FACS. The increase in CD24^hiCD61⁺ luminal progenitor cells is evident in the tumor cells of the MMTV-Wnt/ILK transgenic model compared with MMTV-Wnt (n = 3 per group). (b) Bar chart depicting the relative proportion of the CD61⁺ luminal progenitor cells to the total luminal population (CD29^loCD24^hi) in the two tumor types. Error bars indicate mean ± SD. **P < 0.002 was calculated by using a paired two-sample t test. (c) The purity of the cell populations (myoepithelial and luminal) in the sorted fractions is shown by the lineage-specific markers, K18 and K14, in the cultured tumor cells of both transgenic models. Scale bars, 25 μm.