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Figure 4 | Breast Cancer Research

Figure 4

From: Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development

Figure 4

Biochemical analysis, densitometry results, and immunochemistry. (a) The biochemical analysis of the tumors from each group demonstrates upregulation of certain molecular markers in MMTV-Wnt/ILK tumors, which could potentially lead to the more-aggressive growth phenotype observed in these tumors. The lysates from two different FVB mammary glands were used as controls. The blots were generated at the same time, by using the same apparatus, and under identical exposure times and conditions to ensure comparability. (b) The graph for the Western-blot analysis illustrates the densitometry results of the blots. Results represent the mean of densitometry data from 10 independent tumors normalized for β-actin expression. The error bars represent the standard error of the mean (SEM). *P ≤ 0.05; **P < 0.005; calculated by using an ANCOVA analysis. (c) β-catenin immunohistochemistry shows a significant increase in nuclear localization in excised tumors from MMTV-Wnt/ILK mice, suggesting a more-active β-catenin-mediated transcriptional machinery. Magnification: (a,b) 40×; (c,d) 63×; n = 4. β-catenin staining of the normal FVB mammary gland is included as a control (e,f). Scale bars, 25 μm. (d) Positive nuclear β-catenin staining was determined by counting the cells that showed overlapping hematoxylin and β-catenin staining. The cells were counted in five equal randomly chosen fields of view at 40× magnification in four individual tumors. Results are reported as the mean of the ratio of the total number of the nuclear-positive cells to the total cell count. The error bars represent the standard error of the mean (SEM). **P < 1.1 × 10-7 was calculated by using a paired two-sample t test.

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