Figure 1

Dose curve and time course of P4's action on epithelial-mesenchymal transition relevant events of MB468 and MB231 cells. The growth-arrested MB468 cells were treated with different doses of progesterone (P4) for (a and c) 24 hours or with P4 at (b) 30 ng/ml for various times as indicated. Western blot analyses were performed with diverse antibodies as indicated. The expression of snail was inhibited by P4 treatments in dose- and time-dependent manners (Figures 1a and b); and the expression of fibronectin, E-cadherin, and occludin was also modulated by P4 in dose dependent manners. (d) Morphological changes of MB468 cells treated with/without P4 at 30 ng/ml for 48 hours. Photos (DIC images) were taken by using confocal microscopy at 400× magnification. (e) The growth-arrested MB231 cells were treated with different doses of P4 for 24 hours as indicated. Western blot analyses were performed with anti-snail and anti-GAPDH antibodies. Equivalent levels of snail expression were shown after the P4 treatment at different doses as indicated. All data were collected and averaged from three individual experiments and the graphs are expressed as fold change over basal. * P < 0.05 for difference of protein expression induced by P4 vs. vehicle alone.