Figure 1

PC-PLC expression on plasma membrane of breast cancer cells. (a) CLSM analyses of six unfixed cell lines stained for PC-PLC detection (pseudo-color grey). Upper panels show examples of a three-dimensional reconstruction of a single cell (scale bars, 5 μm); the lower panels show single central sections of a group of cells (scale bars, 20 μm). Nuclei were stained with DAPI (blue). (b, c) FACS analyses of unfixed cells: representative cytofluorimetric histograms of PC-PLC relative fluorescence intensity of the indicated cell lines (b) and percentage (mean ± SD) of PC-PLC positive cells (c). Statistical significance of differences between HER2-overexpressing (SKBr3 and MDA-MB-453) and HER2-non-overexpressing (MDA-MB-231, MCF-7, MDA-MB-435) carcinoma cell lines was P < 0.001. Five independent experiments were performed on each cell line.