The expression level of HJURP has an independent prognostic impact and predicts the sensitivity to radiotherapy in breast cancer

Table 2 Additional cell line growth conditions and subtypes

Lane	Name	Subtype*	Medium	Culture condition
30	184A1N4	N	MEGM^a	37°C, 5% CO₂
31	184B5	N	MEGM^a	37°C, 5% CO₂
36	HCC1599	Basal A	RPMI1640+10%FBS^b	37°C, 5% CO₂
48	HCC1395	Basal B	RPMI1640+10%FBS	37°C, 5% CO₂
50	HCC1806	Basal A	RPMI1640+10%FBS	37°C, 5% CO₂
53	HCC2218	Luminal	RPMI1640+10%FBS	37°C, 5% CO₂
54	HCC1599	Basal A	RPMI1640+10%FBS	37°C, 5% CO₂
55	UACC893	Luminal	DMEM+10% FBS	37°C, 5% CO₂
56	SUM229PE	N/A	Ham's F12+5% FBS+IH^c	37°C, 5% CO₂
57	MX-1	N/A	RPMI1640+10%FBS	37°C, 5% CO₂
58	SUM102PT	Basal A	Ham's F12+IHE^d	37°C, 5% CO₂
60	HCC1419	Luminal	RPMI1640+10%FBS	37°C, 5% CO₂
62	S1	N	H14 medium +10 ng/ml EGF	37°C, 5% CO₂
63	T4	Basal B	H14 medium^e	37°C, 5% CO₂
64	MDAMB231-Gray	Basal B	DMEM+10% FBS	37°C, 5% CO₂
65	MDAMB231-ATCC	Basal B	DMEM+10% FBS	37°C, 5% CO₂

^a: Clonetics MEBM (no Bi Carbonate)+Insulin(5 ug/ml)+Transferrin(5 ug/ml)+Hydrocortisone(0.5 ug/ml) +EGF(5 ng/ml) +Isoprorternol
^b: Fetal bovine serum (FBS)
^c: Ham's F12 + 5% FBS + IH {insulin (5 ug/ml) + Hydrocortisone (1 ug/ml) + HEPES (10 mM)}
^d: Ham's F12 + IHE {insulin (5 ug/ml) + HEPES (10 mM) + EGF (10 ng/ml)}
^e: H14 medium: DMEM/F12 (GIBCO/BRL) with 250 ng/ml insulin, 10 μg/ml transferrin, 2.6 ng/ml sodium selenite, 10^-10 M estradiol, 1.4 × 10^-6 M
*: N: non-malignant(immortalized), N/A: no data

ISSN: 1465-542X