Figure 7

The HJURP mRNA level in breast cancer cell lines predicts the sensitivity to radiation treatment. Part (a) shows the percent of viable cells at 72 hours after different doses of radiation in breast cancer cell line MDAMB231 with a high level of HJURP and T47D with a low level of HJURP. MDAMD231 cells are more sensitive to radiation treatment than T47D cells. Figure 7b shows the fold change of apoptosis in comparison to control (no radiation) at 72 hours after the different dose of radiation in breast cancer cell line MDAMB231 and T47D. There are more apoptosis in MDAMB231 cells than T47D cells. Part (c) shows the percent of viable cells at 72 hours after the different dose of radiation in breast cancer cell line BT20 with high level of HJURP and MCF10A with low level of HJURP. BT20 cells are more sensitive to radiation treatment than MCF10A cells. Part (d) shows the fold change of apoptosis in comparison to control (no radiation) at 72 hours after the different dose of radiation in breast cancer cell line BT20 and MCF10A. There are more apoptosis in BT20 cells than MCF10A cells. (e) HJURP protein levels are down-regulated by shRNA in MDAMB231 breast cancer cell lines. Part (f) shows that MDAMB231 breast cancer cells with shRNA against HJURP reduce the sensitivity to radiation.