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Table 1 Overview of proteins showing significant differences (P < 0.05) between control and treated MDAMB468 and MDAMB231 breast cancer cells

From: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

MDAMB468 cells

Control vs. lactone

Control vs. acid

14-3-3 beta

3.51

1.93

14-3-3 zeta/theta

1.61

1.48

17-beta hydroxysteroid dehydrogenase

0.62

0.47

6-phosphogluconolactonase

1.56

1.5

alpha enolase

1.89

1.37

alpha glucosidase subunit alpha isoform 3

0.64

0.4

ATPase, H+ transporting, lysosomal 70 kDa V1 subunit A

0.30

0.31

ATP citrate lyase beta, mitochondrial

0.33

0.63

Carbonyl reductase [NADPH] 3

3.00

1.56

Chaperonin containing TCP1, subunit 6A

1.34

1.98

chloride intracellular channel 1

1.2

2.12

cofilin 1/2

0.60

0.65

D3-phosphoglycerate dehydrogenase

0.75

0.16

DJ-1

0.54

0.45

EEF1 delta

0.68

0.4

ER-60 protein

2.18

1.56

eukaryotic initiation factor 4A-II

0.35

0.63

eukaryotic translation initiation factor 3 subunit H

0.71

0.49

eukaryotic translation initiation factor 6

4.76

1.57

Ezrin

2.10

4.00

gelsolin precursor

2.73

1.54

glutamate receptor GRIA3

0.24

0.63

glycyl-tRNA synthetase

0.40

0.33

heat shock cognate 71 kDa protein

0.20

0.36

IMMT (mitochondrial inner membrane protein)

3.44

2.32

lamin A/C, isoform CRA_c

0.41

0.47

MAPRE1 protein

0.86

0.4

multidrug resistance-associated protein MGr1-Ag

0.52

0.74

NADH dehydrogenase Fe-S protein 1, 75 kDa

0.56

0.35

nucleoside phosphorylase

1.95

1.31

protein disulfide isomerase associated 6

0.85

0.41

protein disulfide isomerase ER-60

2.13

1.25

RAB8b, member RAS oncogene family

3.16

1.48

RAVER-1 protein

2.17

1.60

splicing factor, arginine/serine-rich 1

0.24

0.71

sterol carrier protein X/2

0.45

0.52

stress-70 protein, mitochondrial precursor

(observed pI 5.9; theoretical pI 5.9)

0.65

0.57

stress-70 protein, mitochondrial precursor

(observed pI 5.6; theoretical pI 5.9)

0.59

0.42

succinate dehydrogenase [ubiquinone] flavoprotein subunit

0.59

0.3

triosephosphate isomerase

(pI observed 6.7; theoretical pI 6.5)

0.70

0.64

thioredoxin domain-containing protein 12

0.64

0.7

RhoA precursor

0.65

0.6

MDAMB231 cells

  

3-hydroxyisobutyrate dehydrogenase, mitochondrial

0.64

0.73

aldose reductase

1.64

1.40

alpha enolase

0.28

absent

annexin A1

(observed pI 6.6; theoretical pI 6.6)

1.67

1.42

annexin A1

(observed pI 6.4; theoretical pI 6.6)

1.24

3.08

annexin A4

1.52

1.63

cathepsin D precursor

0.55

0.52

cell division cycle protein 42

1.76

2.04

chloride intracellular channel protein 1

(observed pI 5.1; theoretical pI 5.1)

1.58

1.98

chloride intracellular channel protein 1

(observed pI 5.3; theoretical pI 5.1)

1.61

1.97

Cleavage stimulation factor 64 kDa subunit

0.28

0.35

cofilin 1/2

0.62

0.71

complement component 1 Q

subcomponent-binding protein, mitochondrial

0.41

0.63

Cytochrome b-c1 complex subunit 1, mitochondrial

0.71

0.63

Cytochrome c-type heme lyase

0.35

0.37

dihydrolipoamide S-acetyltransferase, component of PDH complex

0.60

0.68

DJ-1

0.34

0.44

elongation factor 1-delta

0.85

0.24

endoplasmic reticulum protein ERp29 precursor

1.36

1.60

eukaryotic translation initiation factor 3 subunit I

0.70

0.73

Ezrin

2.31

1.77

GDP dissociation inhibitor 2

1.6

1.54

gelsolin precursor (identified in 3 spots as fragment)

1.77

1.80

glutathione S-transferase Pi

1.56

1.84

glutathione S-transferase omega-1

1.65

1.57

glycyl-tRNA synthetase

1.91

1.41

GrpE protein homolog 1, mitochondrial precursor

0.67

0.76

heat shock protein 27

1.33

1.57

heterogeneous nuclear ribonucleoprotein F

0.72

0.95

heterogeneous nuclear ribonucleoprotein H1

1.91

2.32

heterogeneous nuclear ribonucleoprotein K

0.41

0.24

heterogeneous nuclear ribonucleoproteins C1/C2

0.56

0.91

high mobility group protein B1

2.59

1.94

interferon-induced GTP-binding protein Mx2

1.24

1.59

Ku70 antigen

0.46

0.35

lactoyl-glutathione lyase

1.82

2.02

lamin-A/C

0.36

0.45

LIM and SH3 domain protein 1

1.73

1.75

macrophage-capping protein (identified in two spots)

2.00

1.98

minichromosome maintenance protein 7

0.44

0.28

moesin

0.53

1.02

MutS homolog 2

0.35

0.16

nucleophosmin

0.61

0.22

peroxiredoxin 2

1.56

1.58

peroxiredoxin 3

1.63

1.81

plexin-D1 precursor

0.30

0.53

pre-mRNA-processing factor 19

0.76

0.48

prohibitin

1.75

1.59

proliferating cell nuclear antigen

0.15

0.49

proteasome activator complex subunit 1

1.20

1.40

proteasome activator complex subunit 3

0.49

0.39

protein NDRG1

1.69

1.58

putative ATP-dependent Clp protease

proteolytic subunit, mitochondrial

0.59

0.76

Ran-specific GTPase-activating protein

1.54

1.66

Ras GTPase-activating protein-binding protein 1

0.54

0.48

reticulocalbin-1 precursor

(identified in two spots as fragment)

0.47

0.64

reticulocalbin-1 precursor

0.58

0.78

stomatin-like protein 2

0.64

0.52

stress-70 protein, mitochondrial

(observed pI 5.9; theoretical pI 5.9)

0.61

0.74

stress-70 protein, mitochondrial precursor

(observed pI 5.4; theoretical pI 5.9)

0.59

0.42

synaptic vesicle membrane protein VAT-1 homolog

5.14

4.55

RhoA precursor

0.49

0.65

TRAP1

1.54

1.76

triosephosphate isomerase

(pI observed 6.2; theoretical pI 6.5)

2.37

2.14

triosephosphate isomerase

(pI observed 6.5; theoretical pI 6.5)

0.77

0.59

tropomyosin 1 alpha chain isoform 4

0.31

0.44

vinculin

2.12

2.69

zyxin

2.39

0.82

  1. The cell treatment occurred with either 8 μg/mL lovastatin lactone or hydroxy acid for 48 hours. The factor change is presented below with values above 1 representing an increase and values below 1 representing a decrease in protein expression as compared with controls. In some cases (annexin 1, chloride intracellular channel protein 1, stress-70 protein, triose phosphate isomerase) more then one spot was assigned to one protein. This happens when proteins undergo a post-translational modification as indicated by a shift in the spot's isoelectric point (pI). In these cases, an observed and a theoretical pI values were provided.