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Table 1 Overview of proteins showing significant differences (P < 0.05) between control and treated MDAMB468 and MDAMB231 breast cancer cells

From: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

MDAMB468 cells Control vs. lactone Control vs. acid
14-3-3 beta 3.51 1.93
14-3-3 zeta/theta 1.61 1.48
17-beta hydroxysteroid dehydrogenase 0.62 0.47
6-phosphogluconolactonase 1.56 1.5
alpha enolase 1.89 1.37
alpha glucosidase subunit alpha isoform 3 0.64 0.4
ATPase, H+ transporting, lysosomal 70 kDa V1 subunit A 0.30 0.31
ATP citrate lyase beta, mitochondrial 0.33 0.63
Carbonyl reductase [NADPH] 3 3.00 1.56
Chaperonin containing TCP1, subunit 6A 1.34 1.98
chloride intracellular channel 1 1.2 2.12
cofilin 1/2 0.60 0.65
D3-phosphoglycerate dehydrogenase 0.75 0.16
DJ-1 0.54 0.45
EEF1 delta 0.68 0.4
ER-60 protein 2.18 1.56
eukaryotic initiation factor 4A-II 0.35 0.63
eukaryotic translation initiation factor 3 subunit H 0.71 0.49
eukaryotic translation initiation factor 6 4.76 1.57
Ezrin 2.10 4.00
gelsolin precursor 2.73 1.54
glutamate receptor GRIA3 0.24 0.63
glycyl-tRNA synthetase 0.40 0.33
heat shock cognate 71 kDa protein 0.20 0.36
IMMT (mitochondrial inner membrane protein) 3.44 2.32
lamin A/C, isoform CRA_c 0.41 0.47
MAPRE1 protein 0.86 0.4
multidrug resistance-associated protein MGr1-Ag 0.52 0.74
NADH dehydrogenase Fe-S protein 1, 75 kDa 0.56 0.35
nucleoside phosphorylase 1.95 1.31
protein disulfide isomerase associated 6 0.85 0.41
protein disulfide isomerase ER-60 2.13 1.25
RAB8b, member RAS oncogene family 3.16 1.48
RAVER-1 protein 2.17 1.60
splicing factor, arginine/serine-rich 1 0.24 0.71
sterol carrier protein X/2 0.45 0.52
stress-70 protein, mitochondrial precursor
(observed pI 5.9; theoretical pI 5.9)
0.65 0.57
stress-70 protein, mitochondrial precursor
(observed pI 5.6; theoretical pI 5.9)
0.59 0.42
succinate dehydrogenase [ubiquinone] flavoprotein subunit 0.59 0.3
triosephosphate isomerase
(pI observed 6.7; theoretical pI 6.5)
0.70 0.64
thioredoxin domain-containing protein 12 0.64 0.7
RhoA precursor 0.65 0.6
MDAMB231 cells   
3-hydroxyisobutyrate dehydrogenase, mitochondrial 0.64 0.73
aldose reductase 1.64 1.40
alpha enolase 0.28 absent
annexin A1
(observed pI 6.6; theoretical pI 6.6)
1.67 1.42
annexin A1
(observed pI 6.4; theoretical pI 6.6)
1.24 3.08
annexin A4 1.52 1.63
cathepsin D precursor 0.55 0.52
cell division cycle protein 42 1.76 2.04
chloride intracellular channel protein 1
(observed pI 5.1; theoretical pI 5.1)
1.58 1.98
chloride intracellular channel protein 1
(observed pI 5.3; theoretical pI 5.1)
1.61 1.97
Cleavage stimulation factor 64 kDa subunit 0.28 0.35
cofilin 1/2 0.62 0.71
complement component 1 Q
subcomponent-binding protein, mitochondrial
0.41 0.63
Cytochrome b-c1 complex subunit 1, mitochondrial 0.71 0.63
Cytochrome c-type heme lyase 0.35 0.37
dihydrolipoamide S-acetyltransferase, component of PDH complex 0.60 0.68
DJ-1 0.34 0.44
elongation factor 1-delta 0.85 0.24
endoplasmic reticulum protein ERp29 precursor 1.36 1.60
eukaryotic translation initiation factor 3 subunit I 0.70 0.73
Ezrin 2.31 1.77
GDP dissociation inhibitor 2 1.6 1.54
gelsolin precursor (identified in 3 spots as fragment) 1.77 1.80
glutathione S-transferase Pi 1.56 1.84
glutathione S-transferase omega-1 1.65 1.57
glycyl-tRNA synthetase 1.91 1.41
GrpE protein homolog 1, mitochondrial precursor 0.67 0.76
heat shock protein 27 1.33 1.57
heterogeneous nuclear ribonucleoprotein F 0.72 0.95
heterogeneous nuclear ribonucleoprotein H1 1.91 2.32
heterogeneous nuclear ribonucleoprotein K 0.41 0.24
heterogeneous nuclear ribonucleoproteins C1/C2 0.56 0.91
high mobility group protein B1 2.59 1.94
interferon-induced GTP-binding protein Mx2 1.24 1.59
Ku70 antigen 0.46 0.35
lactoyl-glutathione lyase 1.82 2.02
lamin-A/C 0.36 0.45
LIM and SH3 domain protein 1 1.73 1.75
macrophage-capping protein (identified in two spots) 2.00 1.98
minichromosome maintenance protein 7 0.44 0.28
moesin 0.53 1.02
MutS homolog 2 0.35 0.16
nucleophosmin 0.61 0.22
peroxiredoxin 2 1.56 1.58
peroxiredoxin 3 1.63 1.81
plexin-D1 precursor 0.30 0.53
pre-mRNA-processing factor 19 0.76 0.48
prohibitin 1.75 1.59
proliferating cell nuclear antigen 0.15 0.49
proteasome activator complex subunit 1 1.20 1.40
proteasome activator complex subunit 3 0.49 0.39
protein NDRG1 1.69 1.58
putative ATP-dependent Clp protease
proteolytic subunit, mitochondrial
0.59 0.76
Ran-specific GTPase-activating protein 1.54 1.66
Ras GTPase-activating protein-binding protein 1 0.54 0.48
reticulocalbin-1 precursor
(identified in two spots as fragment)
0.47 0.64
reticulocalbin-1 precursor 0.58 0.78
stomatin-like protein 2 0.64 0.52
stress-70 protein, mitochondrial
(observed pI 5.9; theoretical pI 5.9)
0.61 0.74
stress-70 protein, mitochondrial precursor
(observed pI 5.4; theoretical pI 5.9)
0.59 0.42
synaptic vesicle membrane protein VAT-1 homolog 5.14 4.55
RhoA precursor 0.49 0.65
TRAP1 1.54 1.76
triosephosphate isomerase
(pI observed 6.2; theoretical pI 6.5)
2.37 2.14
triosephosphate isomerase
(pI observed 6.5; theoretical pI 6.5)
0.77 0.59
tropomyosin 1 alpha chain isoform 4 0.31 0.44
vinculin 2.12 2.69
zyxin 2.39 0.82
  1. The cell treatment occurred with either 8 μg/mL lovastatin lactone or hydroxy acid for 48 hours. The factor change is presented below with values above 1 representing an increase and values below 1 representing a decrease in protein expression as compared with controls. In some cases (annexin 1, chloride intracellular channel protein 1, stress-70 protein, triose phosphate isomerase) more then one spot was assigned to one protein. This happens when proteins undergo a post-translational modification as indicated by a shift in the spot's isoelectric point (pI). In these cases, an observed and a theoretical pI values were provided.