Western blot analysis of proteins involved in regulation of apoptosis and AKT-signaling. Breast cancer cell lines MDAMB231 and MDAMB468 were treated with 8 μg/mL lovastatin lactone (Lova Lac) or lovastatin acid (Lova Ac) for 48 hours. For key proteins, western blot analysis of phosphatase and tensin homolog (PTEN), pAkt and N-myc downstream regulated gene 1 (NDRG1) was performed based on MDAMB231 cell extracts, otherwise both cell lines are shown. Densitometry data were normalized based on the amount of β-actin. Data are presented as means ± standard deviations (n = 3) *P < 0.05;**P < 0.05; ***P < 0.001). Gel images were cropped to improve the clarity and conciseness of the presentation. PCNA, proliferating cell nuclear antigen.