Effects of ABL-N on the expression of Bcl-2 family. (a) Treatment of ABL-N decreased the level of the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic proteins Bax and Bad in MDA-MB-231 cells. (b) Effect of ABL-N on the Bax/Bcl-2 ratio in MDA-MB-231 cells. The data obtained from the Western blot analysis of Bax and Bcl-2 were used to evaluate the effect of ABL-N on the Bax/Bcl-2 ratio. The densitometric analysis of Bax and Bcl-2 bands was performed using TotalLab TL120 software, and the data (relative density normalized to β-actin) were plotted as Bax/Bcl-2 ratio. Data are expressed as means ± SE of three separate experiments with similar results. *P <0.05, **P <0.01, as compared with the group without ABL-N treatment.