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Figure 2 | Breast Cancer Research

Figure 2

From: (-)-Epigallocatechin gallate sensitizes breast cancer cells to paclitaxel in a murine model of breast carcinoma

Figure 2

EGCG potentiates taxol-induced apoptosis in 4T1 cells. (a) 4T1 cells were treated with 20 μM EGCG, 1 μM taxol, 10 μM SP600125 or combination for 24 h, and apoptosis was assessed by Hoechst 33342 staining. The apoptotic cells with strong fluorescence or fragmented nuclei were observed under fluorescent microscopy. (b) Quantification of apoptotic cells was performed by taking the images in random fields and counting cells with strong fluoresence or fragmented nuclei. The relative apoptosis rate was plotted. Columns = mean percentage of apoptotic cells; bars = SE. Asterisk = P < 0.001 versus untreated control or taxol in combination with 10 μM SP600125. Black triangle = P < 0.001 versus EGCG, taxol, or taxol in combination with EGCG and SP600125. (c) 4T1 cells were treated with 20 μM EGCG, 1 μM taxol, EGCG in combination with taxol, taxol in combination with 10 μM SP600125, or taxol in combination with EGCG and SP600125 for 24 h. Total proteins were harvested with lysis buffer containing phosphatase inhibitor, and subjected to Western blot analysis of JNK phosphorylation. The ratio of p-JNK/actin as determined by densitometric analysis was given. (d) 4T1 cells were treated with 20 μM EGCG, 1 μM taxol, or EGCG in combination with taxol for 24 h. Total proteins were harvested and subjected to Western blot analysis of GRP78 expression. The ratio of GRP78/actin as determined by densitometric analysis was given. Representative of two experiments was shown.

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