Figure 1

EGCG sensitizes breast cancer cells to taxol. (a) Sensitivity of 4T1, MDA-MB-231 and MCF-7 cells to taxol. Cells were treated with increasing doses of taxol (0.1 to 2 μM) for 24 h. Cell viability was assessed by WST1 assay. Points = mean of four replicates; bars = SE. Asterisk = P < 0.01 versus untreated controls. (b) 4T1 cells were seeded in a 96-well plate at 5,000 cells per well. The next day, the cells were treated with 20 μM EGCG, 1 μM taxol, or a combination for 48 h. Cell viability was assessed by WST1 assay. Points = mean of four replicates; bars = SE. Asterisk = P < 0.01 versus untreated control. Triangle = P < 0.001 versus single agent. (c) MDA-MB-231 cells were seeded in a 96-well plate at 5,000 cells per well. The next day, the cells were treated with 20 μM EGCG, 1 μM taxol, or combination for 48 h. Cell viability was assessed by WST1 assay. Points = mean of four replicates; bars = SE. Asterisk = P < 0.001 versus untreated control. Triangle = P < 0.001 versus single agent.