Expression of PIPKIγ in breast carcinomas. (a) Shown is H&E, E-cadherin and type I gamma phosphatidylinositol phosphate kinase (PIPKIγ) staining of normal breast tissue (top). Breast carcinomas that show a loss of both E-cadherin and membrane PIPKIγ (middle). A fraction of carcinomas that show both a mistargeting of E-cadherin and a loss of PIPKIγ (bottom). (b) Expression of PIPKIγ in breast carcinomas. (Left panel) Representative breast carcinomas stained negative for PIPKIγ. (Middle panel) Representative breast carcinomas stained weakly positive for PIPKIγ. (Right panel) Representative breast carcinomas stained strong positive for PIPKIγ. (c) Immunohistochemical staining of breast tumor tissue for human epidermal growth factor receptor (HER-1) and PIPKIγ. The panels on the top or on the bottom are from the same tumors. The top panels show strong expression of HER-1 and PIPKIγ, whereas the bottom shows weak staining for both antigens.