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Table 3 Description of normal, premalignant, and malignant breast tissues utilized for MSP analysis along with methylation status of RASSF1A and SFRP1 in each tissue

From: Human mammary cancer progression model recapitulates methylation events associated with breast premalignancy

Sample Description RASSF1A SFRP1
N1 Normal reduction mammoplasty tissue U U
N2 Normal reduction mammoplasty tissue U U
N3 Normal reduction mammoplasty tissue M M
N4 Normal reduction mammoplasty tissue U U
N5 Normal reduction mammoplasty tissue U U
N6 Normal reduction mammoplasty tissue U U
N7 Normal reduction mammoplasty tissue U U
Total Normal
Methylated
  1/7 1/7
H1 Lobular hyperplasia (85% of mammary cells), blunt duct hyperplasia (15% of mammary cells) U U
H2 Normal (80%), intraductal hyperplasia (10%), cystic hyperplasia (10%) U U
Total Hyperplasia
Methylated
  0/2 0/2
H3 Focal atypical terminal duct hyperplasia (single focus); fibrocystic disease M M
Total ADH
Methylated
  1/1 1/1
D1 CCL, DCIS M M
D2 DCIS M M
D3 DCIS, cysts, normal breast tissue M M
D4 DCIS and normal tissue U M
D5 Extensive comedo DCIS, calcifications M M
D6 Extensive DCIS M M
D7 Extensive DCIS and normal tissue M M
D8 DCIS, calcifications U M
D9 Extensive DCIS M M
D10 DCIS with calcifications M M
D11 Extensive comedo DCIS M M
Total DCIS
Methylated
  9/11 11/11
C1 IDC, grade 2, node-, ER+ M M
C2 IDC, grade 3, node+, ER- M M
C3 IDC, grade 3, node+, ER- U M
C4 IDC, grade 3, node-, ER- M M
C5 IDC, grade 3, node+, ER+ M M
C6 IDC, grade 1, node+, ER+ M M
C7 IDC, grade 3, node+, ER- M M
C8 IDC, DCIS, CCL, normal breast M M
C9 IDC, DCIS M M
C10 IDC, DCIS, normal breast U M
C11 IDC and extensive DCIS M M
C12 IDC and extensive comedo DCIS with calcifications M M
Total IDC
Methylated
  10/12 12/12
  1. ADH = atypical ductal hyperplasia; CCL = columnar cell lesion; DCIS = ductal carcinoma in situ; IDC = invasive ductal carcinoma; ER = estrogen receptor; U = unmethylated; M = methylated. For SFRP1, methylation status of the CpG island located in exon 1 is shown.