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Table 1 MSP primers

From: Human mammary cancer progression model recapitulates methylation events associated with breast premalignancy

Gene Name Forward Primer (5'-3') Reverse Primer (5'-3') Product size (bp) Primer location relative to TSS (bp) Ref
p16INK4A-U TTA TTA GAG GGT GGG GTG GAT TGT CAA CCC CAA ACC ACA ACC ATA A 151 +132 to 283 [22]
p16INK4A-M TTA TTA GAG GGT GGG GCG GAT CGC GAC CCC GAA CCG CGA CCG TAA 150 +132 to 282  
HOXA9-U TTG GGG TTA GAT AGG GAG TTG GGA AAA AAT AAA AAC AAA AAA CAA ACA AA 167 -4453 to -4286 [16]
HOXA9-M TCG GGG TTA GAT AGG GAG TCG GGA AAA ATA AAA ACG AAA AAC AAA CGA A 166 -4453 to -4287  
CCND2-U AGA GTA TGT GTT AGG GTT GAT T ACA TCC TCA CCA ACC CTC CA 108 -1167 to -1059 [12]
CCND2-M CGG CGA TTT TAT CGT AGT CG CTC CAC GCT CGA TCC TTC G 101 -1138 to -1037  
RASSF1A-U TTT GGT TGG AGT GTG TTA ATG TG CAA ACC CCA CAA ACT AAA AAC AA 108 +201 to +309 [23]
RASSF1A-M GTG TTA ACG CGT TGC GTA TC AAC CCC GCG AAC TAA AAA CGA 94 +213 to +307  
SFRP1-U* GTT TTG TAG TTT TTG GAG TTA GTG TTG TGT CTC AAC CTA CAA TCA AAA ACA ACA CAA ACA 135 -2 to +133 [24]
SFRP1-M TGT AGT TTT CGG AGT TAG TGT CGC GC CCT ACG ATC GAA AAC GAC GCG AAC G 126 +2 to +128  
SFRP1-U** TTT TAG TAA ATT GAA TTT GTT TGT GA TAA AAT ACA CAA AAC TGG TAG AAC 149 -151 to -2 [40]
SFRP1-M TTT AGT AAA TCG AAT TCG AAT TCG TTC GC TAA AAT ACG CGA AAC TCC TAC G 148 -150 to -2  
p57-U GTT GTT TGT GTT TGT GTA GTT TT AAA AAT CCC ACA AAC AAC AAA ACA 91 +248 to +339  
p57-M TTG TTC GCG TTT GCG TAG TTT C AAA TCC CAC GAA CGA CAA AAC G 88 +249 to +337  
MGMT-U TTT GTG TTT TGA TGT TTG TAG GTT TTT GT AAC TCC ACA CTC TTC CAA AAA CAA AAC A 93 +30 to +123 [25]
MGMT-M TTT CGA CGT TCG TAG GTT TTC GC GCA CTC TTC CGA AAA CGA AAC G 81 +36 to +117  
THBS1-U TTG AGT TTG TGT GGT GTA AGA GTA T CCC CAC TAC CTA ACA CAC AAC T 156 -280 to -124 [26]
THBS1-M GTT CGC GTG GCG TAA GAG TAC CGC TAC CTA ACG CGC AAC T 149 -276 to -127  
  1. *exon 1
  2. **promoter