Hypoxia-inducible factor-1α and vascular endothelial growth factor expression in circulating tumor cells of breast cancer patients

Table 2 VEGF mRNA expression in CTCs of patients with metastatic breast cancer

No Patients	Ct _(CTCs)	Ct _(PBMCs)	2^-ΔCt
1	32,6	30,6	0,3
2	33,7	36,8	8,3
3	24,1	28,7	25,3
4	37,9	38,8	1,8
5	41,0	41,0	1,0
6	39,4	41,0	3,1
7	34,8	32,3	0,2
8	41,0	36,9	0,1
9	35,7	32,2	0,1
10	34,7	38,2	11,5

A 2^-ΔCt method was used to examine the relative expression of VEGF in CTCs-enriched fraction compared to the CTCs-depleted fraction. Equal RNA amount from each fraction was processed for RT-PCR and Real-Tme PCR. Ct is the Real-Time PCR sample threshold cycle and ΔCt is (Ct_(CTCs)- Ct_(PBMCs)), PBMCs are the Peripheral Blood Mononuclear Cells (the CTCs-depleted fraction).

ISSN: 1465-542X