|
No Patients
|
Ct (CTCs)
|
Ct (PBMCs)
|
2-ΔCt
|
|---|
|
1
|
32,6
|
30,6
|
0,3
|
|
2
|
33,7
|
36,8
|
8,3
|
|
3
|
24,1
|
28,7
|
25,3
|
|
4
|
37,9
|
38,8
|
1,8
|
|
5
|
41,0
|
41,0
|
1,0
|
|
6
|
39,4
|
41,0
|
3,1
|
|
7
|
34,8
|
32,3
|
0,2
|
|
8
|
41,0
|
36,9
|
0,1
|
|
9
|
35,7
|
32,2
|
0,1
|
|
10
|
34,7
|
38,2
|
11,5
|
- A 2-ΔCt method was used to examine the relative expression of VEGF in CTCs-enriched fraction compared to the CTCs-depleted fraction. Equal RNA amount from each fraction was processed for RT-PCR and Real-Tme PCR. Ct is the Real-Time PCR sample threshold cycle and ΔCt is (Ct(CTCs)- Ct(PBMCs)), PBMCs are the Peripheral Blood Mononuclear Cells (the CTCs-depleted fraction).
