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Table 2 VEGF mRNA expression in CTCs of patients with metastatic breast cancer

From: Hypoxia-inducible factor-1α and vascular endothelial growth factor expression in circulating tumor cells of breast cancer patients

No Patients Ct (CTCs) Ct (PBMCs) 2-ΔCt
1 32,6 30,6 0,3
2 33,7 36,8 8,3
3 24,1 28,7 25,3
4 37,9 38,8 1,8
5 41,0 41,0 1,0
6 39,4 41,0 3,1
7 34,8 32,3 0,2
8 41,0 36,9 0,1
9 35,7 32,2 0,1
10 34,7 38,2 11,5
  1. A 2-ΔCt method was used to examine the relative expression of VEGF in CTCs-enriched fraction compared to the CTCs-depleted fraction. Equal RNA amount from each fraction was processed for RT-PCR and Real-Tme PCR. Ct is the Real-Time PCR sample threshold cycle and ΔCt is (Ct(CTCs)- Ct(PBMCs)), PBMCs are the Peripheral Blood Mononuclear Cells (the CTCs-depleted fraction).