Skip to main content
Figure 1 | Breast Cancer Research

Figure 1

From: Ku proteins interact with activator protein-2 transcription factors and contribute to ERBB2overexpression in breast cancer cell lines

Figure 1

Identification of the Ku proteins interacting with AP-2α. (a) Two-dimensional gel electrophoresis of nuclear proteins eluted from GST AP-2α coated beads or beads coated with GST alone (GST). The gels were silver stained and the proteins retained on GST AP-2α beads were identified by mass spectrometry. Arrows show the location of Ku80 (up) and Ku70 (down). Molecular masses are indicated on the left and the pH scale on the top of the gels. (b) The presence of AP-2α and Ku proteins in the eluted fractions was verified by immunoblotting (IB) and compared to a fraction of the input (IN). (c) Co-immunoprecipitation (IP) of AP-2 and Ku proteins by AP-2, Ku70 and Ku80 antibodies or an Ig control (Ig Ctl). The proteins were revealed by immunoblotting (IB).

Back to article page

Breast Cancer Research

ISSN: 1465-542X

Contact us