Depletion of CD24 caused increased invasiveness without yielding a mesenchymal phenotype. (a) Ca1a cells were transfected with non-targeting or CD24 siRNA. Histogram illustrates CD24-PE fluorescence intensity of unstained cells (black line) and cells transfected with CD24 siRNA (red line) or non-targeting siRNA (blue line) as assessed by flow cytometric analysis. (b) Total RNA was isolated from cells following transfection with non-targeting or CD24-targeting siRNA. Transcript abundance was evaluated via realtime RT-PCR. Non-targeting siRNA transfected cell (blue bars) and CD24-targeting siRNA transfected cell (red bars) differences are presented using the delta delta Ct method. Graphs represent means and associated standard errors of experiments performed in triplicate. * indicates P < 0.05. (c), 24 hr following transfection with either non-targeting or CD24 siRNA, Ca1a cells were trypsinized, counted, and seeded to Matrigel invasion chambers in triplicate. Invaded cells were counted 48 hr later. Graph represents mean fold change in invaded cells associated with CD24 siRNA transfection and associated standard errors. * indicates P < 0.05.