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Figure 3 | Breast Cancer Research

Figure 3

From: Dynamic regulation of CD24 and the invasive, CD44posCD24negphenotype in breast cancer cell lines

Figure 3

Clones generated from a single CD44posCD24pos or CD44posCD24neg cell possess molecular and functional heterogeneity similar to that of parental line. (a) Clones were generated by sorting single Ca1a CD44posCD24pos or CD44posCD24neg cells into individual wells of 96-well plates. Only wells confirmed to contain a single cell were expanded. (b) CD44/CD24 expression of representative clones derived from a single CD44posCD24neg or CD44posCD24pos cell. From the experiment described in (a), seven clones were generated from sorted CD44posCD24pos cells and five clones were generated from CD44posCD24neg cells. The CD44/CD24 profiles of representative clones are presented. (c) Total RNA was isolated from isogenic CD44posCD24neg and CD44posCD24pos cells sorted from the clones described in (a, b) and transcript abundance was evaluated via realtime RT-PCR. Data generated with cells sorted from a clone derived from a single CD44posCD24neg cell are presented in red. Data generated with cells sorted from a clone derived from a single CD44posCD24pos cell are presented in blue. CD44posCD24neg (closed bars) and CD44posCD24pos (open bars) differences are presented using the delta delta Ct method. Graphs represent means and associated standard errors of three independent experiments. * indicates P < 0.05. (d) Invasion through Matrigel by isogenic CD44posCD24neg and CD44posCD24poscells freshly sorted from the clones described in (a, b, c). 48 hr following seeding, invaded cells were counted. Graphs represent averages and associated standard errors of experiments performed in triplicate. Data generated with cells sorted from a clone derived from a single CD44posCD24neg cell are presented in red. Data generated with cells sorted from clone derived from a single CD44posCD24pos cell are presented in blue. * indicates P < 0.05.

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