Figure 3

Nonlinear association between TPH1 and cancer progression. (A-B) Results depicting quantification of TPH1 immunostaining from blindly scored tissue microarray sections. Data were sorted based on the indicated criteria. Data were analyzed by one-way ANOVA with P-values as noted in comparison with NI for (A) and N0 for (B). Error bars represent ± SEM. (A) TPH1 scores in samples identified as non-invasive (NI); invasive, node-negative (IN-); invasive, node-positive (IN+); and invasive with distant metastases (IN-Mets). (B) Human breast cancer tissue sections scored for TPH1 were sorted as per different stages of nodal metastasis (N-stage: N0, no regional lymph node involvement; N1, Mets to movable ipsilateral nodes; N1a/b, Mets up to 20 mm to 4 or more nodes with extension beyond node capsule; N2-3, Mets to ipsilateral nodes that are fixed to one another or to other structures and to internal mammary lymph nodes). (C) Representative microarray sections stained for TPH1 from sorted groups as indicated below each image. Brown/red dye indicates TPH1 stain, blue is hematoxylin.