Expression of Hes-6 is increased in breast cancer. (a) Expression of Hes-6 mRNA in breast cancer cell lines T47D (bar 1), MCF-7 (bar 2), SKBR3 (bar 3), and MDA-MB-231 (bar 4). All experiments were repeated twice with similar results. Data are presented as mean values of six samples (bars 1, 2, and 3) and five samples (bar 4) ± SEM. Expression of Hes-6 mRNA (b) and E2F-1 mRNA (c) in noncancerous breast biopsies (bar 1) compared with malignant breast (bar 2). Data are presented as mean values of 24 (bars 1) and 28 (bars 2) samples ± SEM. Graphs are shown as relative values to bars 1 (100%) of each graph. Significance is presented as *P < 0.05; **P < 0.005; ***P < 0.001. Nonsignificant differences are presented as NS.