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Table 1 Summary of massively parallel sequencing technologies

From: Next-generation sequencing

Method Amplification Read length (base pairs) Templates per run Data production/day Sequence reaction Reference
Commercially available technologies
   ABI 3730xI PCR ~900 to 1,100 96 1 Mb/day Sanger method http://www.appliedbyosystems.com
   454 FLX Roche Emulsion PCR ~400 1,000,000 400 Mb/run/7.5 to 8 hours Pyrosequencing http://www.rocheapplied-science.com
   Illumina (Solexa) Genome Analyzer Bridge PCR 36 to 175 40,000,000 >17 Gb/run/3 to 6 days Reverse terminator http://www.illumina.com
   ABI SOLiD Emulsion PCR ~50 85,000,000 10 to 15 Gb/run/6 days Ligation sequencing http://www.appliedbyosystems.com
   Helicos Heliscope None 30 to 35 800,000,000 21 to 28 Gb/run/8 days Single molecule sequence by synthesis http://www.helicosbio.com
Technologies in development
   Pacific Biosciences None >1,000 NA NA Single molecule real-time DNA sequencing http://www.pacificbiosciences.com
   Intelligent Biosciences Yesa NA NA NA Sequence by synthesis http://www.intelligentbiosystems.com
   Visigen Biotechnologies None NA NA NA Base-specific FRET emission http://www.visigenbio.com
   ZS Genetics None NA NA NA ZSG atomic labelling and electron microscopy http://www.zsgenetics.com
  1. NA, not available at present. aAmplification method not yet standardised. FRET, Förster resonance energy transfer.