An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ

Behbod, Fariba; Kittrell, Frances S; LaMarca, Heather; Edwards, David; Kerbawy, Sofia; Heestand, Jessica C; Young, Evelin; Mukhopadhyay, Purna; Yeh, Hung-Wen; Allred, D Craig; Hu, Min; Polyak, Kornelia; Rosen, Jeffrey M; Medina, Daniel

doi:10.1186/bcr2358

Breast Cancer Research

Table 1 Transplant results

From: An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ

Cell line	Group	Number of positive fps/total	Ratio of positive growth	Total number of ducts positive	Number of tumors
DCIS.COM	Random sort	9/28	32%	22+	1
DCIS.COM	CD49f^hi/MUC-1^med	4/23	17%	11+
DCIS.COM	CD49f^hi/MUC-1^lo	4/6	67%	9
DCIS.COM	CD49f^med/MUC-1^lo	4/23	17%	8
DCIS.COM	CD49f^med/MUC-1^med	4/6	67%	15
DCIS.COM	CD49f^hi/CD24^med	3/8	38%	4
DCIS.COM	CD49f^hi/CD24^lo	5/8	63%	18
DCIS.COM	CD44^med/CD24^med	3/24	13%	3
DCIS.COM	CD44^med/CD24^lo	1/6	17%	1
DCIS.COM	CD44^hi/CD24^lo	7/24	29%	26	3
DCIS.COM	CD44^hi/CD24^med	3/6	50%	5
SUM-225	Random sort	1/16	6%	7+
SUM-225	CD49f^med/MUC-1^hi	6/11	55%	31+
SUM-225	CD49f^med/MUC-1^med	0/6	0%	0
SUM-225	CD49f^lo/MUC-1^med	1/12	8%	2
SUM-225	CD49f^lo/MUC-1^hi	0/6	0%	0
SUM-225	CD49f^med/CD24^hi	4/6	67%	22+
SUM-225	CD49f^med/CD24^med	0/6	0%	0
SUM-225	CD44^lo/CD24^hi	0/6	0%	0
SUM-225	CD44^med/CD24^med	2/6	33%	8+

A fixed number of cells (DCIS.COM = 5,000 cells in 2 μl and SUM-225 = 10,000 cells in 2 μl) sorted into different subpopulations or randomly sorted followed by injection into primary mammary ducts of SCID-beige female mice. Random sort refers to cells sorted based on forward and side scatter and irrespective of the expression of specific markers. Six weeks after intraductal injections, glands were removed, subjected to whole-mount staining, and the numbers of glands containing positive growth (DCIS-like lesions) were counted. The fraction of growth is referred to the number of fat pads containing positive DCIS-like lesions over the total number of fat pads transplanted. The columns contain the following data: Column 1 (Cell line): Cell line injected; Column 2 (Group): Subpopulations sorted and injected intraductally; Column 3 (Number of positive fps/total): Number of fat pads (fps or glands) containing positive growth (DCIS-like lesions) over the total number of fat pads (glands) transplanted; Column 4 (Ratio of positive growth): Fraction of fat pads containing positive growth (DCIS-like lesions); Column 5 (Total number ducts positive): Total number of positive ducts (ducts containing DCIS-like lesions) combined in all the positive glands per group (plus sign indicates one or more fat pads contained more than seven positive ducts); Column 6 (Number of tumors): Number of palpable tumors formed; these data were not included in the statistical evaluation. In DCIS.COM, the expression levels of the basal markers ranged from med to hi, and expression levels of luminal markers ranged from lo to med. Therefore, DCIS.COM does not contain CD49f^lo-, CD44^lo-, CD24^hi-, or MUC-1^hi-expressing cells. In SUM-225, the expression levels of basal markers CD49f and CD44 ranged from lo to medium, and the expression levels of the luminal markers CD24 and MUC-1 ranged from med to hi. Therefore, the groups do not include CD49f^hi-, CD44^hi-, CD24^lo-, and MUC-1^lo-expressing cells.

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ISSN: 1465-542X

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