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Table 1 Transplant results

From: An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ

Cell line Group Number of positive fps/total Ratio of positive growth Total number of ducts positive Number of tumors
DCIS.COM Random sort 9/28 32% 22+ 1
DCIS.COM CD49fhi/MUC-1med 4/23 17% 11+  
DCIS.COM CD49fhi/MUC-1lo 4/6 67% 9  
DCIS.COM CD49fmed/MUC-1lo 4/23 17% 8  
DCIS.COM CD49fmed/MUC-1med 4/6 67% 15  
DCIS.COM CD49fhi/CD24med 3/8 38% 4  
DCIS.COM CD49fhi/CD24lo 5/8 63% 18  
DCIS.COM CD44med/CD24med 3/24 13% 3  
DCIS.COM CD44med/CD24lo 1/6 17% 1  
DCIS.COM CD44hi/CD24lo 7/24 29% 26 3
DCIS.COM CD44hi/CD24med 3/6 50% 5  
SUM-225 Random sort 1/16 6% 7+  
SUM-225 CD49fmed/MUC-1hi 6/11 55% 31+  
SUM-225 CD49fmed/MUC-1med 0/6 0% 0  
SUM-225 CD49flo/MUC-1med 1/12 8% 2  
SUM-225 CD49flo/MUC-1hi 0/6 0% 0  
SUM-225 CD49fmed/CD24hi 4/6 67% 22+  
SUM-225 CD49fmed/CD24med 0/6 0% 0  
SUM-225 CD44lo/CD24hi 0/6 0% 0  
SUM-225 CD44med/CD24med 2/6 33% 8+  
  1. A fixed number of cells (DCIS.COM = 5,000 cells in 2 μl and SUM-225 = 10,000 cells in 2 μl) sorted into different subpopulations or randomly sorted followed by injection into primary mammary ducts of SCID-beige female mice. Random sort refers to cells sorted based on forward and side scatter and irrespective of the expression of specific markers. Six weeks after intraductal injections, glands were removed, subjected to whole-mount staining, and the numbers of glands containing positive growth (DCIS-like lesions) were counted. The fraction of growth is referred to the number of fat pads containing positive DCIS-like lesions over the total number of fat pads transplanted. The columns contain the following data: Column 1 (Cell line): Cell line injected; Column 2 (Group): Subpopulations sorted and injected intraductally; Column 3 (Number of positive fps/total): Number of fat pads (fps or glands) containing positive growth (DCIS-like lesions) over the total number of fat pads (glands) transplanted; Column 4 (Ratio of positive growth): Fraction of fat pads containing positive growth (DCIS-like lesions); Column 5 (Total number ducts positive): Total number of positive ducts (ducts containing DCIS-like lesions) combined in all the positive glands per group (plus sign indicates one or more fat pads contained more than seven positive ducts); Column 6 (Number of tumors): Number of palpable tumors formed; these data were not included in the statistical evaluation. In DCIS.COM, the expression levels of the basal markers ranged from med to hi, and expression levels of luminal markers ranged from lo to med. Therefore, DCIS.COM does not contain CD49flo-, CD44lo-, CD24hi-, or MUC-1hi-expressing cells. In SUM-225, the expression levels of basal markers CD49f and CD44 ranged from lo to medium, and the expression levels of the luminal markers CD24 and MUC-1 ranged from med to hi. Therefore, the groups do not include CD49fhi-, CD44hi-, CD24lo-, and MUC-1lo-expressing cells.