Hematoxylin and eosin (H&E) and whole-mount staining of intraductal xenografts. The panels show whole-mount and H&E staining of xenografts generated by intraductal transplantation of DCIS.COM (a, b), SUM-225 (c, d), and FSK-H7 (e, f). The H&E figures depict ×20 magnification, and whole mounts were captured at ×1.6 (SUM-225 and DCIS.COM) and ×0.7 (FSK-H7). Cells were injected intraductally (20,000/μl cells in 2 μl of PBS) into the primary mammary ducts (via cleaved nipple) of intact 8-week-old immunocompromised SCID-beige female mice. The human primary DCIS was enzymatically digested into single cells before injections. The pictures were taken at 6 weeks after injection.