BRCA1-deficient mammary tumor cells are dependent on EZH2 expression and sensitive to Polycomb Repressive Complex 2-inhibitor 3-deazaneplanocin A

Table 2 IC₅₀ values of DZNep on BRCA1-deficient vs. BRCA1-reconstituted mammary tumor cells

Cell line	nM DZNep (SEM)
*BRCA1-proficient*
KP-6.3	3523 (463)
*BRCA1-reconstituted*
KB1PR-3.12 E3	1954 (744)
KB1PR-3.12 F4	829 (331)
*BRCA1-deficient*
KB1P-3.12	135 (13)
KB1P40.1	187 (33)
Ratio (BRCA1-reconst. vs. deficient)	8.7*
Significance	0.003

Note: Values between brackets represent the standard error of the mean (SEM) of at least three independent experiments. Ratio: Average half maximal inhibitory concentration (IC₅₀) values of 3-deazaneplanocin A (DZNep) for the K 14cre; B rca 1^F/F; p 53^F/F(KB1P) lines were compared with average IC₅₀ values of the KB1P lines. *Significance: P < 0.01 (t-test).

ISSN: 1465-542X