Figure 3

Haploinsufficiency for p190B blocks preneoplastic progression by reducing angiogenesis. (a) Mammary tissues from tumor burden mice with the indicated genotypes were examined by whole-mount analyses. Arrows, preneoplasias detected. LN, lymph node. Scale bar = 500 μm. (b) Number of preneoplastic lesions in one Number 4 inguinal gland per tumor-burden mouse was quantified. Student's t test showed significant difference between groups (P < 0.04). (c) Gene expression qRT-PCR data from Superarray angiogenesis array. Array analysis revealed a twofold increase in thrombospondin-1 (TBS-1) in the p190B^+/-Neu mammary glands of adult mice 8 to 12 weeks of age. (d) Immunohistochemistry to detect TBS-1 protein demonstrated that TBS-1 expression is localized to the mammary epithelium and is increased in p190B^+/-Neu mammary glands (upper) as compared with wild-type glands (lower). Images shown are at 400× magnification. (e) Quantification of TBS-1 immunostaining showed that TBS-1 protein is increased fourfold in p190B^+/-Neu mammary glands. Error bars, standard error of the mean.