Figure 7From: Construction of an MUC-1 promoter driven, conditionally replicating adenovirus that expresses the sodium iodide symporter for gene therapy of breast cancerAnalysis of sodium iodide symporter (NIS) expression. (a) NIS expression assayed with Western blotting in Ad5AMUCH_RSV-NIS-infected cells. Membrane proteins were prepared 24 hours after infection. (b) Radioiodine uptake dose-response. T47D cells were infected at the indicated multiplicity of infection (MOI). Radioiodine uptake was measured 24 hours after infection. (c) Kinetics of NIS-mediated radioiodine uptake. T47D cells were infected at MOI 20 with the Ad5AMUCH_RSV-NIS conditionally replicating adenovirus (CRAd), and 125I uptake was measured daily.Back to article page