Protein | HER2 EC | phospho-HER2 (pY1248) | p27Kip1 | Oestrogen receptor α | Progesterone receptor |
---|---|---|---|---|---|
Antibody | Rabbit mAb | Mouse mAb | Mouse mAb | Mouse mAb | Mouse mAb |
Origin | Thermo Scientific | Thermo Scientific | Lab Vision | Novocastra | Novocastra |
Clone | SP3 | PN2A, Ab-18 | DCS-72.F6, Ab-1 | 6F11 | 1A6 |
Dilution of primary antibody | 1:50 | 1:100 | 1:100 | 1:60 | 1:40 |
Staining pattern | Membrane | Nucleus and cytoplasm | Nucleus | ||
Epitope retrieval | Heat induced, 10 mM citric acid buffer, pH 7.3 | Heat induced, 10 mM sodium-citric acid pH 6.0 | Heat induced, 10 mM citric acid buffer, pH 7.3 | ||
Blocking | Endogenous peroxidase blocking | ||||
Primary antibody | 30 min, room temperature | Overnight, 4°C | 30 min, room temperature | 60 min, room temperature | |
Detection system | EnVision™ Dual Link System; DAB + chromogenic substrate | iVIEW™ DAB Detection Kit | |||
Scoring in accordance with | HercepTest guidelines | Immunoreactive score | Manufacturer's guidelines |