The correlation of c-Src activity with an EMT. (a) Nuclear pStat-3 (blue) in invasive lobular carcinoma (ILC) cells with activated c-Src (red). Note the weak nuclear Stat-3 staining in lobular carcinoma in situ (LCIS; asterisk). An antibody against total Stat-3 was used as a control. (b) Left panel: widespread Stat-3 activation in ILC cells. Middle panel: increased levels of activated Fak in ILC cells surrounding nonneoplastic epithelium and LCIS (asterisks). Right panel: expression of pFak in LCIS (asterisk) and ILC (right). An antibody against total Fak was used as a control. (c) Colocalization of active c-Src (green) and active Fak (red) in ILC cells. (d) ILC cells with activated c-Src (green) are positive for the mesenchymal marker vimentin (red). (e) Expression of the mesenchymal N-cadherin (green) in a subset of invasive cancer cells.