Figure 5
From: The role of YY1 in reduced HP1α gene expression in invasive human breast cancer cells
YY1 occupancy at the HP1α gene promoter is higher in MCF7 cells than in HS578T cells. (a) Chromatin immunoprecipitation (ChIP) assay was performed using chromatin form MCF7 and HS578T cells. Chromatin was immunoprecipitated using the antibodies indicated above each lane. PCR reactions were specific for the region indicated to the left of each panel. A region 2.2 kb upstream of the glucocorticoid receptor (GR) gene transcriptional start site was used as a positive control for ying yang 1 (YY1) binding. A region 22 kb downstream from the HP1α start site was used as a negative control. YY1 occupancy at the HP1α promoter can be observed only in the MCF7 samples. YY1 occupancy at the GR gene upstream regulatory region can be observed in both cell lines. (b) Quantification of ChIP data from at least three independent experiments using different chromatin preparations. NRF-1 = nuclear respiratory factor-1.