Immunohistochemistry panel showing differential expression pattern of FOXA1 and GATA-3. An example of luminal A (L1 to L7), basal-like (B1 to B7) and human epidermal growth factor receptor 2 (HER-2)-overexpressing (H1 to H7) invasive breast tumours. Expression of the most commonly used breast cancer markers is also illustrated for comparison with the forkhead box A1 (FOXA1) and GATA binding protein 3 (GATA-3) expression. (L1, B1, H1) Haematoxylin-eosin stainings from each of the selected core cases. (L3, L4) Strong and restricted nuclear expression of FOXA1 and GATA-3 in the normal breast duct (internal control) and in the luminal A invasive tumour (grade II). (B3, B4) Negative expression of FOXA1 and GATA-3 in basal subtype tumour (grade III). (H3, H4) HER-2-overexpressing tumour showing negativity for FOXA1 and GATA-3 expression (grade III). All microscopy images are at 40× magnification. ER, oestrogen receptor; P-CAD, P-cadherin; CK, cytokeratin.