Figure 3

WNT promotes MDA-MB-231 cell migration. (a) Confluent monolayers of MDA-MB-231/secreted Frizzled-related protein 1 (sFRP1)-P1 cells and control-P1 cells were scratched, the medium was changed to Wnt1 conditioned medium (CM) or control CM, and 3 hours later the recovered areas were calculated on nine randomly chosen wound edges. Results presented in arbitrary units. Average recovered areas calculated ± standard error of the mean (SEM). **P < 0.01, n.s. = not significant (P > 0.05). (b) MDA-MB-231 cells were treated with 100 ng/ml recombinant Wnt3a for the indicated time. Active β-catenin was detected by western analysis using specific antiserum; α-tubulin level serves as a loading standard. (c) Confluent monolayers of MDA-MB-231 cells cultured in DMEM, 10% FCS were changed to DMEM, 10% FCS with or without 100 ng/ml recombinant Wnt3a, and 90 minutes later the monolayer surface was scratched. The recovered area was measured on nine randomly chosen wound edges, 3 and 6 hours later. y axis, recovered area (arbitrary unit) ± SEM. **P < 0.01.