Protein expression in HC11 stem-like, pre-differentiated and differentiated cells. (a) Melk expression analyzed by western blot and immunofluorescence. (b) Dusp 6 expression by western blot. (c) COUPTF-II expression by western blot and immunofluorescence. (d) ADAMTS1 expression analyzed by western blot. *Processed forms of the zymogen. In all cases, whole cell extracts were resolved on 10% SDS-PAGE and membranes blotted with the indicated antibodies. Tubulin was used as loading control.