Figure 5

Activation of iFGFR1 induces Cox-2 expression in vivo, which is regulated in part by IL-1β activity. (a) Cyclooxygenase (Cox) 2 immunohistochemistry of mammary gland sections from either non-transgenic or transgenic mice treated with AP20187 for either 48 hours or 4 weeks. (b) Cox-2 immunohistochemistry of mammary gland sections from transgenic mice treated with either an isotype goat IgG or a neutralising IL-1β antibody in conjunction with AP20187 treatment. Magnification is 50 μM. The figure is representative of sections from three mice per treatment. (c) Percentage of epithelial cells expressing Cox-2 in panels a and b. Error bars represent standard error of the mean. * P < 0.05, ** P < 0.01. (d) Quantitative RT-PCR was used to analyse Cox-2 expression in mammary glands from non-transgenic and transgenic mice following treatment with AP20187 and either IgG or IL-1β blocking antibody. Cox-2 expression was normalised to cyclophilin expression. Error bars represent standard error of the mean. * P < 0.05, ** P < 0.01. Ab = antibody; IHC = immunohistochemistry; MMTV = mouse mammary tumour virus; NF = nuclear factor; iFGFR1 = inducible fibroblast growth factor receptor 1.