Figure 2

Analysis of mammary glands from iFGFR1 transgenic mice following activation of iFGFR1 and treatment with an IL-1β neutralising antibody. H&E-stained sections from (a) non-treated control mice, (b) transgenic mice treated with AP20187 and goat IgG, and (c) transgenic mice treated with AP20187 and IL-1β blocking antibody (Ab). Arrows indicate epithelial budding structures. Scale bar = 100 μM. (d) Budding structures were quantified by counting epithelial structures in H&E-stained sections (three mice per treatment group). Decreased epithelial budding was observed following treatment with AP20187 and the IL-1β antibody compared with treatment with AP20187 and IgG. ** P < 0.01 (e) Sections were immunostained with an antibody to phospho-histone H3 (pH3), a marker of mitosis, and the percentage of pH3 positive epithelial cells was determined. Decreased numbers of pH3 were observed following treatment with AP20187 and the IL-1β antibody compared with treatment with AP20187 and IgG. * P < 0.05, ** P < 0.01. iFGFR1 = inducible fibroblast growth factor receptor 1.