Figure 3

Isolation of total epithelial cell populations from mammary epithelium. (a) Whole mount of mammary fat pad transplanted with primary cells transduced with green fluorescent protein (GFP) lentivirus. Arrow, area enlarged in inset. Bar = 2.5 mm. (b) Flow cytometric analysis of transplanted epithelium. The histogram indicates levels of GFP fluorescence in cells isolated from transplanted mammary fat pads. The CD24 Sca-1 staining pattern of the total cells (GFP^- and GFP⁺) harvested from the fat pads is shown. The CD24 Sca-1 staining patterns of the GFP^- and GFP⁺ cells are also shown separately. GFP⁺ cells, which must represent the progeny of transduced stem cells, are found only in the CD24^+/Low Sca-1^-, CD24^+/High Sca-1^- and CD24^+/High Sca-1⁺ regions. Data are representative of four independent experiments.