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Figure 4 | Breast Cancer Research

Figure 4

From: Loss of TGF-β or Wnt5a results in an increase in Wnt/β-catenin activity and redirects mammary tumour phenotype

Figure 4

Sca1, a marker of mammary stem/progenitor cells, is increased in DNIIR and Wnt5a-/- glands. mRNA and protein were extracted from several wild type (WT) and MT-DNIIR (DNIIR) mice. (a) Sca1 mRNA levels were determined by semi-quantitative RT-PCR and (b) protein levels were determined by western blot. Sca1 levels were increased in DNIIR glands relative to controls. (c) Sca1 protein levels were determined in WT and Wnt5a null (-/-) glands using western blot analysis. Sca1 levels were increased in Wnt5a-null tissue relative to controls. 18S and β-Tubulin (β-Tub) were used as normalisation controls. DNIIR = dominant-negative transforming growth factor-β type II receptor; RT-PCR = reverse transcripase polymerase chain reaction; Wnt = Wingless-related protein family.

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