Figure 5
ERBB2-positive cells' sensitivity to palmitate is through ROS formation and ceramide production. (a) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0, 0.5, and 1 μmol/l of N-acetyl-cysteine (NAC) for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. ***P < 0.05. (b) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0 and 10 μmol/l of the ceramide synthesis inhibitor fumonisin B1 for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. ROS, reactive oxygen species.