Palmitate toxicity of ERBB2-positive cells is due to increased apoptosis. (a) Images of BT474, MDA-MB-361, MCF-7, and human mammary epithelial cell (HMEC) cells stained for activated Bax signal that were treated with 500 or 750 μmol/l palmitate, 20 μmol/l GW9662, or 500 μmol/l palmitate plus 20 μmol/l GW9662, all for 72 hours. Hoechst 33243 was used for nuclear staining. (b, c) Quantification of activated Bax signal. Error bars indicate the standard deviation from three individual experiments. ***P < 0.004, **P = 0.01.