Figure 6
From: Methylation of homeobox genes is a frequent and early epigenetic event in breast cancer
Bisulfite sequencing data. The extent of CpG methylation was determined for target sequences within (a) CGI 7:48, (b) HOXB13, (c) HNF1B, and (d) HLXB9 by sodium bisulfite sequencing. Primary breast tumors were subjected to bisulfite DNA sequencing together with their matching normal counterpart (ductal carcinoma in situ case number 57 and stage I, case numbers 4b and 233). Sequencing results of several independent clones are shown. Black circles, methylated CpG dinucleotides. Open brackets indicate the location of the BstUI restriction sites in the target sequence.